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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has actually been noticed in lots of tumors, which leads to chromosome instability, and thus may well lead to tumorigenesis. This protein was observed to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a critical purpose in endorsing mobile-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are actually described. Two more splice variants were noted but specific nucleotide sequence info is not yet available.
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Blocking peptides are peptides that bind exclusively to your concentrate on antibody and block antibody binding. These peptide usually consists of the epitope acknowledged because of the antibody.
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This guideline outlines more doable optimization of mobile and immunoassay parameters to make sure the absolute best success are...
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This information outlines more probable optimization of mobile and immunoassay parameters to be certain the best possible results are...
Antibodies bound to the blocking peptide not bind towards the epitope around the target protein. By evaluating the staining from your blocked antibody compared to the antibody by yourself, you can see which staining is distinct.
Target Information and facts Cyclin E belongs to your highly conserved cyclin relatives, whose associates are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit unique expression and degradation styles which lead on the temporal coordination of every mitotic occasion. Cyclin E types a complex with and capabilities being a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S changeover. This protein accumulates on the G1-S phase boundary and is particularly degraded as cells progress via S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both equally antibodies...
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